Customers talk about us

GeneCellin HTC
"I tested the transfection efficiency of GeneCellin HTC in comparison to a range of other transfection reagents...I achieved a relatively high transfection efficiency of cells that were previously difficult to transfect. I have re-ordered this product on several occasions, the reagent has arrived promptly and gave consistently reproducible results." C. McFarlane PhD, Queen's University of Belfast.

"The aim of my work is to transfect cells with a plasmidic antagomiR to silence the activity of a specific miRNA. I recommand this reagent since until now, all my transfections in Human Keratinocytes were successful (almost 70% of transfection efficiency) with almost no damage on my cells viability." G. Degueurce, UNIL, Lausanne.

Welcome to BioCellChallenge

BioCellChallenge is an innovating company that creates, develops and markets drug delivery systems. Our expertise allows to provide very efficient and reproducible transfection reagents to the scientific community. Our reagents are home-made and unique on the market. 0ur scientists remain at your disposal to discuss any scientific topic with you.

You are looking for a specific lipid that you can't find anywhere...
We offer an innovative custom lipid synthesis service. Our ten years experience in this field will guarantee you high quality results.

Worldwide Distribution

We are recruiting distributors in different countries. Contact us
  Distributors
 
We welcome Bulldog Bio as our new distributor for USA/Canada.

BioCellChallenge

BioCellChallenge SAS
Parc d'Activités de Signes
Avenue de Paris
BP90778
83030 Toulon cedex 9 (France)

NucleoBooster Transfection Reagent Enhancer

    

NucleoBooster Transfection Reagent Enhancer


NucleoBooster is a unique formulation which enhances transfection efficiency of plasmid DNA into cultured cells. Its compatibility has been successfully tested with most of well-known commercialy available lipid-based transfection reagents. NucleoBooster increases transfection efficiencies without modifying any characteristics and cell specificities of your regular transfection reagent. Simple and rapid to use, NucleoBooster is compatible with any transfection protocol.

 

  Enhancer Transfection Reagent      Enhancer Transfection Reagent

  Enhancer Transfection Reagent      Enhancer Transfection Reagent

  Without NucleoBooster      With NucleoBooster     

  

Main advantages

Increase transfection efficiencies

Compatible with all lipid-based transfection reagents

Not toxic

Just mix NucleoBooster with DNA prior transfection

No optimization required

BEAS-2B and MDCK cells were transfected with a GFP-encoding plasmid and a well known lipid-based transfection reagents according to manufacturer's protocols (left pictures). The same transfections were also performed by using NucleoBooster at 2 µL / µg of plasmid DNA. NucleoBooster was mixed with plasmid DNA before performing transfections (right pictures). Efficiencies were evaluated 24 h after transfections under an epifluorescent microscope

 

Enhancer Transfection Reagent

 

 Ordering information
 Reference  Size

Number of transfection

in a 24-well plate

  Prices
 NB500  500 µL  250  contact us
 NB1000  1000 µL  500
 
 

Several cell lines were transfected with a well-know lipid-based transfection reagent (Reagent A) according to manufacturer's protocol. For each cell line, transfections were performed with or without NucleoBooster. When NucleoBooster was used, GFP encoding plasmid was mixed with it prior to transfections. Transfection efficiencies were evaluated 48 h post transfections by using a cytofluorimeter.