3D Cell Transfection Kit

Add a New Dimension to Your Transfection

The 3D Cell Transfection Kit is based on a unique in vitro transfection technology which allows researchers to achieve high delivery efficiencies of plasmid DNA into 3D cultured cells. This innovative product includes a sterile plate containing 3D Cell Culture Scaffolds (3D Biotek, LLC) and an especially designed 3D Transfection Reagent (BioCellChallenge, SAS). With this kit, researchers can now perform extended 3D transgene expression studies in cells grown in physiological-like tissue environments.

3D Cell Culture Scaffolds are made from polystyrene, the same material as traditional tissue culture plates. The combination of polystyrene’s transparency and the porous structural design of 3D Cell Culture Scaffolds allows researchers to monitor cell growth and transfection efficiency under an inverted light microscope.

3D Cell Culture Scaffolds within the 3D Cell Transfection Kit have increased surface areas compared with 2D cell culture plates. As a result, more cells can be transfected on 3D Cell Culture Scaffolds while using the same size cell culture plates.

3D Cell Culture Scaffolds will not absorb cytokines, growth factors, or any other molecules. Therefore, molecules secreted by the transfected cells can easily be separated or recovered from culture medium without extensive separation steps.

Figure 1: Cells growing on 3D Inserts^TM (3D Biotek, LLC) were transfected with a plasmid DNA encoding GFP and the 3D Transfection Reagent (BioCellChallenge, SAS). Protein expression was monitored by fluorescence microscopy 24h to 72h post transfection.

Fibroblast Cell Transfection

NIH3T3 Cell Transfection

3D Cell Transfection Kit advantages

In vivo-like cell culture environment

High transfection efficiency & reproducibility

Increased protein production

Longer transgene expression

Easy separation of proteins secreted by 3D transfected cells

One step « Transfect & Seed » protocol

Compatible with inverted light microscope

Easy to handle scaffolds

3D Cell Transfection Kit is ideal to transfect

Adherent cell lines

Primary & stem cell culture

It has been well documented that cell culture in tissue culture plates and flasks does not mimic the in vivo cell growth. Therefore, the cell expansion and cell-drug interactions under 2D are not appropriate in vitro models. 3D cell culture technique, on the other hand, offers a better cell culture environment because it is one step closer to the in vivo cell growth environment.

For more information visit our partner website at www.3dbiotek.com

For your convenience, BioCellChallenge SAS distributes polystyrene 3D Cell Culture Scaffolds from 3D Biotek LLC (USA).

3D Cell Culture

Ordering information
Reference	Number of transfection	Prices
3D-DNA24*	12	Price list
3D-DNA96*	24

Related Products
PS152006-3		Price list
PS152012-6
PS152024-12
PS152096-24
TDG100
TDG20

* 3D-DNA24 3D Cell Transfection Kit contains 12 inserts for a 24-well plate (ref. PS152024-12) and 200 µL of 3D Transfection Reagent (ref. TDG200).

* 3D-DNA96 3D Cell Transfection Kit contains 24 inserts for a 96-well plate (ref. PS152096-24) and 100 µL of 3D Transfection Reagent (ref. TDG100).

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