DNA Transfection Reagent

GeneCellin Transfection Reagent

 From Delivery to Discovery

GeneCellinTM is a powerful in vitro transfection reagent (patent pending) which allows to achieve higher delivery efficiencies of plasmid DNA into cell lines and primary cells compare to other lipid and polymer based transfection reagents. This innovative transfection reagent is composed by a stable formulation of nanoparticules we have named “nanoporters”. This technology allows achieving superior and reproducible transgene expression levels without any toxic effect.

 

Cell lines transfection

 Figure 1: GFP expression in various cell lines transfected with 0.5 µg of plasmid DNA and 2 µL of GeneCellinTM Transfection Reagent in a 24-well plate.

 

Hela cell transfection

Epithelial cells transfection

GeneCellin DNA Transfection Reagent advantages

High efficiency & reproducibility

Maximum cell viability

Low amount of DNA required

Serum compatible

Rapid one-tube protocol

 

GeneCellin is ideal to transfect

Adherent & suspension cell lines

Primary cells

Hard-to-transfect cells

 

Applications

Stable & transient transfections

Co-transfections

Reverse transfections

 

 

GeneCellin is a powerful transfection reagent that only requires a small amount of plasmid DNA. Using less DNA reduces adverse cytotoxic effects triggered by transfection and maintains high cell viability with high transfection efficiency.

Figure 2: HeLa cells were transfected with 0.25 µg of plasmid DNA per well in a 24-well plate and several commercial transfection reagents according to the manufacturer's protocols.

 

 Transfection reagent comparison
Cell types

 Transfected cells

%

Cell types

Transfected cells

%

 A549  60  HEK 293  95
 B50  60  HeLa  95
 B65  35  HEPG2  55
 BHK 21  65  L929  60
 Calu-1  70  MCF-7  30
 CHO  90  MDCK  85
 Colo  20  NIH3T3  95
 COS-7  90  Raw 264  60
 HCT-116  75  Vero  50

 

Table 1: Transfection efficiency in different cell lines.

GeneCellin shows very good transfection efficiency in most used cell lines (see  Table 1). GeneCellin was also successfully tested with several primary cells. 

 Ordering information

 Reference  Size

Number of transfection

in a 24-well plate

 Prices
 GC50 -Sample  50 µL  25  Free
 GC500  500 µL  250  contact us
 GC1000  1000 µL  500
 GC5000  5 x 1000 µL  2500

You need help. Do not hesitate to contact us we will provide you all the assistance you need. We guarantee you an answer under 24h.

 

Protocol GeneCellin

MSDS GeneCellin

Results GeneCellin

List of cells GeneCellin

free sample